Ciona robusta (formerly C. intestinalis) as a model of gut host-microbe interactions

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Liberti A, Zucchetti I, Melillo D, Skapura D, Shibata Y, De Santis R, Pinto MR, Litman GW, Dishaw LJ.
Chitin protects the gut epithelial barrier in a protochordate model of DSS-induced colitis. Biol Open. 2018 Jan 17;7(1). pii: bio029355. doi: 10.1242/bio.029355. PubMed PMID: 29222175.
Enter the name for this tabbed section: Ciona
Liberti A, Zucchetti I, Melillo D, Skapura D, Shibata Y, De Santis R, Pinto MR, Litman GW, Dishaw LJ. Chitin protects the gut epithelial barrier in a protochordate model of DSS-induced colitis. Biol Open. 2018 Jan 17;7(1). pii: bio029355. doi: 10.1242/bio.029355. PubMed PMID: 29222175.

Leigh BA, Djurhuus A, Breitbart M, Dishaw LJ. The gut virome of the protochordate model organism, Ciona intestinalis subtype A.
Virus Res. 2017 Nov 15;244:137-146. doi: 10.1016/j.virusres.2017.11.015. [Epub ahead of print] PubMed PMID: 29155033.

Leigh B, Karrer C, Cannon JP, Breitbart M, Dishaw LJ. Isolation and Characterization of a
Shewanella Phage-Host System from the Gut of the Tunicate, Ciona intestinalis. Viruses. 2017 Mar 22;9(3). pii: E60. doi: 10.3390/v9030060. PubMed PMID: 28327522

Leigh BA, Liberti A, Dishaw LJ. Generation of Germ-Free
Ciona intestinalis for Studies of Gut-Microbe Interactions. Front Microbiol. 2016 Dec 27;7:2092. doi:10.3389/fmicb.2016.02092.

Dishaw LJ, Leigh B, Cannon JP, Liberti A, Mueller MG, Skapura DP, Karrer CR, Pinto MR, De Santis R, Litman GW. Gut immunity in a protochordate involves a secreted immunoglobulin-type mediator binding host chitin and bacteria.
Nat Commun. 2016 Feb 15;7:10617.

Liberti A, Leigh B, De Santis R, Pinto MR, Cannon JP, Dishaw LJ, Litman GW. An Immune Effector System in the Protochordate Gut Sheds Light on Fundamental Aspects of Vertebrate Immunity.
Results Probl Cell Differ. 2015;57:159-73.

Dishaw LJ, Cannon JP, Litman GW, Parker W. Immune-directed support of rich microbial communities in the gut has ancient roots. Dev Comp Immunol. 2014 Nov;47(1):36-51.

Liberti A, Melillo D, Zucchetti I, Natale L, Dishaw LJ, Litman GW, De Santis R, Pinto MR. Expression of Ciona intestinalis variable region-containing chitin-binding proteins during development of the gastrointestinal tract and their role in host-microbe interactions. PLoS One. 2014 May 2;9(5):e94984.

Dishaw LJ, Flores-Torres J, Lax S, Gemayel K, Leigh B, Melillo D, Mueller MG, Natale L, Zucchetti I, De Santis R, Pinto MR, Litman GW, Gilbert JA. The gut of geographically disparate Ciona intestinalis harbors a core microbiota.
PLoS One. 2014 Apr 2;9(4):e93386.

Dishaw LJ, Flores-Torres JA, Mueller MG, Karrer CR, Skapura DP, Melillo D, Zucchetti I, De Santis R, Pinto MR, Litman GW. A Basal chordate model for studies of gut microbial immune interactions.
Front Immunol. 2012;3:96. Epub 2012 May 3. PubMed PMID: 22563328; PubMed Central PMCID: PMC3342567.

Dishaw LJ, Giacomelli S, Melillo D, Zucchetti I, Haire RN, Natale L, Russo NA, De Santis R, Litman GW, Pinto MR. A role for variable region-containing chitin-binding proteins (VCBPs) in host gut-bacteria interactions.
Proc Natl Acad Sci U S A. 2011 108(40): 16747-52.
Enter the name for this tabbed section: 2018
Liberti A, Zucchetti I, Melillo D, Skapura D, Shibata Y, De Santis R, Pinto MR, Litman GW, Dishaw LJ. Chitin protects the gut epithelial barrier in a protochordate model of DSS-induced colitis. Biol Open. 2018 Jan 17;7(1). pii: bio029355. doi: 10.1242/bio.029355. PubMed PMID: 29222175.

Enter the name for this tabbed section: 2016-2017
Leigh BA, Djurhuus A, Breitbart M, Dishaw LJ. The gut virome of the protochordate model organism, Ciona intestinalis subtype A. Virus Res. 2017 Nov 15;244:137-146. doi: 10.1016/j.virusres.2017.11.015. [Epub ahead of print] PubMed PMID: 29155033.

Leigh B, Karrer C, Cannon JP, Breitbart M, Dishaw LJ. Isolation and Characterization of a
Shewanella Phage-Host System from the Gut of the Tunicate, Ciona intestinalis. Viruses. 2017 Mar 22;9(3). pii: E60. doi: 10.3390/v9030060. PubMed PMID: 28327522

Leigh BA, Liberti A, Dishaw LJ. Generation of Germ-Free
Ciona intestinalis for Studies of Gut-Microbe Interactions. Front Microbiol. 2016 Dec 27;7:2092. doi:10.3389/fmicb.2016.02092.

Dishaw LJ, Leigh B, Cannon JP, Liberti A, Mueller MG, Skapura DP, Karrer CR, Pinto MR, De Santis R, Litman GW. Gut immunity in a protochordate involves a secreted immunoglobulin-type mediator binding host chitin and bacteria.
Nat Commun. 2016 Feb 15;7:10617.
Enter the name for this tabbed section: 2010-2015

Liberti A, Leigh B, De Santis R, Pinto MR, Cannon JP, Dishaw LJ, Litman GW. An Immune Effector System in the Protochordate Gut Sheds Light on Fundamental Aspects of Vertebrate Immunity. Results Probl Cell Differ. 2015;57:159-73.

Litman GW, Dishaw LJ. Early histocompatibility: color the mechanism green and red. Curr Biol. 2015 Nov 2;25(21):R1042-3. doi:10.1016/j.cub.2015.09.053.

Fahsbender E, Rosario K, Cannon JP, Gulland F, Dishaw LJ, Breitbart M. Development of a Serological Assay for the Sea Lion (Zalophus californianus) Anellovirus, ZcAV.
Nature Sci Rep. 2015 May 12;5:9637. doi: 10.1038/srep09637.

Zhang L, Li L, Guo X, Litman GW, Dishaw LJ, Zhang G. Massive expansion and functional divergence of innate immune genes in a protostome.
Nature Sci Rep. 2015 Mar 3;5:8693. doi: 10.1038/srep08693.

Groer MW, Luciano AA, Dishaw LJ, Ashmeade TL, Miller E, Gilbert JA. Development of the preterm infant gut microbiome: a research priority.
Microbiome. 2014 Oct 13;2:38. doi: 10.1186/2049-2618-2-38.

Dishaw LJ, Cannon JP, Litman GW, Parker W. Immune-directed support of rich microbial communities in the gut has ancient roots. Dev Comp Immunol. 2014 Nov;47(1):36-51.

Liberti A, Melillo D, Zucchetti I, Natale L, Dishaw LJ, Litman GW, De Santis R, Pinto MR. Expression of Ciona intestinalis variable region-containing chitin-binding proteins during development of the gastrointestinal tract and their role in host-microbe interactions. PLoS One. 2014 May 2;9(5):e94984.

Dishaw LJ, Flores-Torres J, Lax S, Gemayel K, Leigh B, Melillo D, Mueller MG, Natale L, Zucchetti I, De Santis R, Pinto MR, Litman GW, Gilbert JA. The gut of geographically disparate Ciona intestinalis harbors a core microbiota.
PLoS One. 2014 Apr 2;9(4):e93386.

Dishaw LJ, Litman GW. Changing views of the evolution of immunity. Front Immunol. 2013 May 21;4:122.

Litman GW, Dishaw LJ. Histocompatibility: clarifying fusion confusion.
Curr Biol. 2013 Oct 21;23(20):R934-5. doi: 10.1016/j.cub.2013.08.048. PubMed PMID: 24156816.

Dishaw LJ, Flores-Torres JA, Mueller MG, Karrer CR, Skapura DP, Melillo D, Zucchetti I, De Santis R, Pinto MR, Litman GW. A Basal chordate model for studies of gut microbial immune interactions.
Front Immunol. 2012;3:96. Epub 2012 May 3. PubMed PMID: 22563328; PubMed Central PMCID: PMC3342567.

Dishaw LJ, Haire RN, Litman GW. The amphioxus genome provides unique insight into the evolution of immunity.
Brief Funct Genomics. 2012 Mar;11(2):167-76. PubMed PMID: 22402506; PubMed Central PMCID: PMC3310213.

Dishaw LJ, Giacomelli S, Melillo D, Zucchetti I, Haire RN, Natale L, Russo NA, De Santis R, Litman GW, Pinto MR. A role for variable region-containing chitin-binding proteins (VCBPs) in host gut-bacteria interactions.
Proc Natl Acad Sci U S A. 2011 108(40): 16747-52.

Dishaw LJ, Mueller MG, Haire RN, Litman GW. Transfection-based genomic readout for identifying rare transcriptional splice variants.
Methods Mol Biol. 2011;748:243-54.

Dishaw LJ, Ota T, Mueller MG, Cannon JP, Haire RN, Gwatney NR, Litman RT, Litman GW. The basis for haplotype complexity in VCBPs, an immune-type receptor in amphioxus.
Immunogenetics. 2010 Sep;62(9):623-31. Epub 2010 Jul 22.

Cannon JP, Dishaw LJ, Haire, RN, Litman RT, Ostrov DA, and Litman GW. 2010. Recognition of additional roles for immunoglobulin domains in immune function. Semin Immunol, 2010 Feb;22(1):17-24. Epub 2009 Dec 8. Review. PubMed PMID: 20004115
Enter the name for this tabbed section: Pre-2010

Dorsey, MJ., A. Petrovic, M.R. Morrow, L.J. Dishaw, J.W. Sleasman. 2009. FOXP3 expression following bone marrow transplantation for IPEX syndrome after reduced-intensity conditioning. Immunol Res, 44(1-3): 179-84.(PMID 19471859)

Dishaw, LJ., Litman, G.W. Invertebrate allorecognition: the origins of histocompatibility. 2009.
Current Biology, Apr 14; 19(7): R286-288.

Dishaw, L.J. M. G. Mueller, N. Gwatney, J. P. Cannon, R. N. Haire, R. T. Litman, C. T. Amemiya, T. Ota, L. Rowen, G. Glusman, G, W. Litman. 2008. Genomic Complexity of the Variable Region-Containing Chitin-Binding Proteins in Amphioxus,
BMC Genetics, Dec 1;9(1):78. (PMID: 19046437).

Zhang, Q., C.M. Zmasek, L.J. Dishaw, M.G. Mueller, Y. Ye, , G.W. Litman, A. Godzik.  2008. Novel genes dramatically alter regulatory network topology in amphioxus.
Genome Biology, 9(8):R123. Epub 2008 Aug4 (PMID: 18680598).

Holland, L.Z. et al (Bf annotation community), 2008. The amphioxus genome illuminates vertebrate origins and cephalochordate biology.
Genome Research, 18(7): 1100-11 (PMID 18562680).

Litman, G.W., L.J. Dishaw, J.P. Cannon, R.N. Haire, J.P. Rast.  2007.  Alternative mechanisms of immune receptor diversity.  Current Opinion in Immunology, 19(5): 526-534.

Litman G.W., J. P. Cannon, L. J. Dishaw, R. N. Haire, D. D. Eason, J. A. Yoder, J. Hernandez Prada, D. A. Ostrov.  2007. Immunoglobulin variable regions in molecules exhibiting characteristics of innate and adaptive immune receptors. Immunologic Research: Perspectives in Immunology. 38(1-3): 294-304.

Dishaw, L.J., M.L. Herrera, and C.H. Bigger. 2006.  Characterization and phylogenetic analysis of a Cnidarian LMP X-like cDNA.   Immunogenetics 58 (5-6): 454-464.

Litman, G.W., J.P. Cannon, and L.J. Dishaw. 2005.  Reconstructing immune phylogeny:  new perspectives,  
Nature Reviews Immunology 5(11): 866-879.

Dishaw, L.J., S. L. Smith, and C. H. Bigger. 2005.  Characterization of a C3-like cDNA in a coral:  phylogenetic implications. 
Immunogenetics  57(7):  535-548.

Kozmik, Z., M. Daube, E. Frei, B. Norman, L. Kos, L. J. Dishaw, M. Noll, and J. Piatigorsky.  2003.  Role of Pax Genes in Eye Evolution:  A Cnidarian PaxB Gene Uniting Pax2 and Pax6 Functions. 
Developmental Cell 5: 773-785. (Nov. Issue: Cover Photo).

Piatigorsky, J., B. Norman, L.J. Dishaw, L. Kos, J. Horwitz, P.J. Steinbach, and Z. Kozmik. 2001. J3-crystallin of the jellyfish lens:  Similarity to saposins. 
Proceedings of the National Academy of Sciences. USA.  98(22): 12362-67.
*This website is not an official University of South Florida website. Only the Dishaw Lab is responsible for its content.

Our research is or has been supported by:
National Science Foundation (currently our phage studies)
National Institutes of Health (our collaboration with Dr. Groer in USF Nursing)
All Children’s Hospital Johns Hopkins Medicine Foundation (several studies)
University of South Florida College of Medicine Deans Office (several early studies in
Ciona)